1 Dietary deficiency and malabsorption are the major causes of folate deficiency in humans.
2 Folate is necessary for normal metabolism, DNA synthesis and red blood cell regeneration. Untreated deficiencies may lead to megaloblastic anaemia.
Since a deficiency of either vitamin B12 or folate can cause megaloblastic anaemia, it is advisable to determine the concentration of both vitamin B12 and folate in order to properly diagnose the etiology of anaemia.